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ghost dye uv 450 viability dye  (Cytek Biosciences)


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    Structured Review

    Cytek Biosciences ghost dye uv 450 viability dye
    Ghost Dye Uv 450 Viability Dye, supplied by Cytek Biosciences, used in various techniques. Bioz Stars score: 95/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ghost+dye+uv+450+viability+dye/Ghost+Dye+UV+450/pmc13011057-68-27-33
    Average 95 stars, based on 82 article reviews
    ghost dye uv 450 viability dye - by Bioz Stars, 2026-11
    95/100 stars

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    Related Articles

    other:

    Article Title: Maternal opioid use disorder and hepatitis C infection in pregnancy reshape the peripheral immune landscape at term
    Article Snippet: The products were run on a 1.5% agarose gel alongside no template controls for the extraction, RT-PCR, and cDNA PCR in-triplicate and imaged with GelRed Nucleic Acid Stain (Biotium, Cat#41003) using the Azure 300 Imager (Azure Biosystems).

    Infection:

    Article Title: Maternal opioid use disorder and hepatitis C infection in pregnancy reshape the peripheral immune landscape at term.
    Article Snippet: The resulting viral cDNA was then amplified with HCV 5′ UTR/partial core specific forward and reverse primers www.thelancet.com Vol 126 April, 2026 3 (HCV_5UTR_1_F: 5′- GTC TAG CCA TGG CGT TAG TAT GAG TG -3’ (TM = 58.8 ◦C, MW = 8057.3 gmol-1) and HCV_5UTR_1_R: 5′- ACA AGT AAA CTC CAC CAA CGA G -3’ (TM = 57.0 ◦C, MW = 7588 gmol-1)) using the Platinum SuperFi II PCR Master Mix (Invitrogen, Cat#12368010). .. Thawed PBMCs from pregnant participants with fullterm, healthy pregnancies or those with OUD with and without HCV infection were thoroughly washed with FACS buffer, and stained with Ghost Dye UV 450 viability dye (Cytek Biosciences) at a ratio of 1:100 diluted in FACS buffer for 30 min at 4 ◦C. .. The products were run on a 1.5% agarose gel alongside no template controls for the extraction, RT-PCR, and cDNA PCR in-triplicate and imaged with GelRed Nucleic Acid Stain (Biotium, Cat#41003) using the Azure 300 Imager (Azure Biosystems).

    FACS:

    Article Title: Maternal opioid use disorder and hepatitis C infection in pregnancy reshape the peripheral immune landscape at term.
    Article Snippet: The resulting viral cDNA was then amplified with HCV 5′ UTR/partial core specific forward and reverse primers www.thelancet.com Vol 126 April, 2026 3 (HCV_5UTR_1_F: 5′- GTC TAG CCA TGG CGT TAG TAT GAG TG -3’ (TM = 58.8 ◦C, MW = 8057.3 gmol-1) and HCV_5UTR_1_R: 5′- ACA AGT AAA CTC CAC CAA CGA G -3’ (TM = 57.0 ◦C, MW = 7588 gmol-1)) using the Platinum SuperFi II PCR Master Mix (Invitrogen, Cat#12368010). .. Thawed PBMCs from pregnant participants with fullterm, healthy pregnancies or those with OUD with and without HCV infection were thoroughly washed with FACS buffer, and stained with Ghost Dye UV 450 viability dye (Cytek Biosciences) at a ratio of 1:100 diluted in FACS buffer for 30 min at 4 ◦C. .. The products were run on a 1.5% agarose gel alongside no template controls for the extraction, RT-PCR, and cDNA PCR in-triplicate and imaged with GelRed Nucleic Acid Stain (Biotium, Cat#41003) using the Azure 300 Imager (Azure Biosystems).

    Staining:

    Article Title: Maternal opioid use disorder and hepatitis C infection in pregnancy reshape the peripheral immune landscape at term.
    Article Snippet: The resulting viral cDNA was then amplified with HCV 5′ UTR/partial core specific forward and reverse primers www.thelancet.com Vol 126 April, 2026 3 (HCV_5UTR_1_F: 5′- GTC TAG CCA TGG CGT TAG TAT GAG TG -3’ (TM = 58.8 ◦C, MW = 8057.3 gmol-1) and HCV_5UTR_1_R: 5′- ACA AGT AAA CTC CAC CAA CGA G -3’ (TM = 57.0 ◦C, MW = 7588 gmol-1)) using the Platinum SuperFi II PCR Master Mix (Invitrogen, Cat#12368010). .. Thawed PBMCs from pregnant participants with fullterm, healthy pregnancies or those with OUD with and without HCV infection were thoroughly washed with FACS buffer, and stained with Ghost Dye UV 450 viability dye (Cytek Biosciences) at a ratio of 1:100 diluted in FACS buffer for 30 min at 4 ◦C. .. The products were run on a 1.5% agarose gel alongside no template controls for the extraction, RT-PCR, and cDNA PCR in-triplicate and imaged with GelRed Nucleic Acid Stain (Biotium, Cat#41003) using the Azure 300 Imager (Azure Biosystems).



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